일부 식물성오일의 Streptococcus mutans와 actobacillus casei 성장에 미치는 영향
The growth inhibitory effect of some vegetable oils on Streptococcus mutans and Lactobacillus casei
김세연, 김한나, 전은주, 김진범, 정승화,
소속 상세정보
김세연 ( Kim Se-Yeon ) - 부산대학교 치의학전문대학원 예방치과학교실
김한나 ( Kim Han-Na ) - 청주대학교 보건과학대학 치위생학과
전은주 ( Jun Eun-Joo ) - 부산대학교 치의학전문대학원 예방치과학교실
김진범 ( Kim Jin-Bom ) - 부산대학교 치의학전문대학원 예방치과학교실
정승화 ( Jeong Seung-Hwa ) - 부산대학교 치의학전문대학원 예방치과학교실
KMID : 0355420160400010024
Abstract
Objectives: The purpose of this study was to evaluate the growth inhibitory effects of some vegetable oils on Streptococcus mutans (S. mutans) and Lactobacillus casei (L. casei).
Methods: Two bacterial strains and 5 kinds of test solutions (3 experimental groups: orange essential oil, olive oil, soybean oil; 1 positive control group: chlorhexidine solution; 1 negative control group: broth medium) were used in this study. S. mutans and L. casei pellets were exposed to 1 ml of one of the test solutions for 1 minute. Then, the treated bacterial cells were incubated in fresh broth medium for 0, 4, 8, 16, and 24 hours. The optical density of the broth medium was measured using an ELISA reader at 620 nm. A nonparametric Kruskal-Wallis test (with Mann-Whitney U tests) was performed to compare the change in optical density between different groups at different time points.
Results: Bacterial growth was significantly inhibited in all experimental groups compared to the negative control group. The growth of L. casei was less affected by experimental oils than that of S. mutans. Or-ange essential oil had the maximum growth inhibitory effect on S. mutans up to 8 hours, similar to that in the positive control group (P<0.01). Experimental oils had greater growth inhibitory effect on L. casei than chlorhexidine solution.
Conclusions: This in vitro study confirmed the growth inhibitory effect of some vegetable oils on S. mutans and L. casei. Rising of the mouth using these vegetable oils is expected to have an anti-plaque effect, but additional clinical studies are needed to confirm this.
키워드
Essential oil; Growth inhibitory effect; Lactobacillus casei; Mouth rinse; Streptococcus mutans; Vegetable oil
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